[IDP] Pro-domain of Subtilisin

• Title:  Backbone dynamics of the natively unfolded pro-peptide of subtilisin by heteronuclear NMR relaxation studies
• Journal: J Biomol NMR. 2001 Jul;20(3):233-49.
• DOI:10.1023/A:1011243116136
• Author: Alexei V. Buevich, Ujwal P. Shinde, Masayori Inouye and Jean Baum
• number of AA: 77
• Sequence:
  AGGSTKEKKYIVGFKQTMSAMSSAKKKDVISQKGGKVEKQ
  FKYVNAAAATLDEKAVKELKKDPSVAYVEEDHIAHEY
• BMRB code: not deposited

HSQC or other spectra

[IDP] HCV NS5A domain 2

• Title: Domain 2 of nonstructural protein 5A (NS5A) of hepatitis C virus is natively unfolded
• Journal: Biochemistry. 2007 Oct 16;46(41):11550-8. Epub 2007 Sep 19.
• DOI:N/A
• Author: Yu Liang, Hong Ye, Cong Bao Kang, and Ho Sup Yoon
• number of AA:96 (240-335)
• Sequence:
  KATCTANHDSPDAELIEANLLWRQEMGGNITRVESENKVV
  ILDSFDPLVAEEDEREVSVPAEILRKSRRFARALPVWARP
  DYNPPLVETWKKPDYEHHHHHH
• BMRB code: 15225

HSQC or other spectra

[IDP] Abeta 40 and Abeta 42

• Title: Aβ42 is More Rigid than Aβ40 at the C Terminus: Implications for Aβ Aggregation and Toxicity
• Journal: JMB,2006 Dec 15;364(5):853-62. Epub 2006 Sep 23
• DOI:doi:10.1016/j.jmb.2006.09.046
• Author: Yilin Yan and Chunyu Wang
• number of AA:40/42
• Sequence: (use Abeta 42) DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
• BMRB code: N/A

HSQC or other spectra

[IDP] HIV-1 TAT

• Title: HIV-1 Tat is a natively unfolded protein: the solution conformation and dynamics of reduced HIV-1 Tat-(1-72) by NMR spectroscopy.
• Journal: J Biol Chem. 2006 Mar 31;281(13):8347-56. Epub 2006 Jan 19
• DOI:10.1074/jbc.M510748200
• Author:Shojania S, O’Neil JD
• number of AA:72
• Sequence: from 1-102, I searched from NCBI [gi:114842135]
  MEPVDPNLEPWKHPGSQPPTACSKCYCKKCCWHCQLCFLK
  KGLGISYGRKKRKHRRGTPQSSKDHQNPIPEQPLPIIRGNQT
  GPKEQKKTVASKAERDLCA
• BMRB code: N/A

HSQC or other spectra

[IDP] human gamma synuclein

• Title: Sensitivity of secondary structure propensities to sequence differences between {alpha}- and {gamma}-synuclein: Implications for fibrillation
• Journal: Protein Science (2006), 15:2795-2804
• DOI:10.1110/ps.062465306
• Author:Joseph A. Marsh, Vinay K. Singh, Zongchao Jia, and Julie D. Forman-Kay
• number of AA:127
• Sequence:
  MDVFKKGFSIAKEGVVGAVEKTKQGVTEAAEKTKEGVMYV
  GAKTKENVVQSVTSVAEKTKEQANAVSEAVVSSVNTVATK
  TVEEAENIAVTSGVVRKEDLRPSAPQQEGEASKEKEEVAE
  EAQSGGD
• BMRB code: 7244

HSQC or other spectra

gS (from Supp. material) or HSQC:

[IDP] MBP –murine myelin basic protein

• Title:MR assignment of an intrinsically disordered protein under physiological conditions: the 18.5 kDa isoform of murine myelin basic protein
• Journal: Biomolecular NMR Assignments
• DOI: 10.1007/s12104-007-9016-1
• Author:David S. Libich, Martine M. Monette, Valerie J. Robertson and George Harauz1
• number of AA:176 (with extra H6 in C-term)
• Sequence:
  ASQKRPSQRSKYLATASTMDHARHGFLPRHRDTGILDSIG
  RFFSGDRGAPKRGSGKDSHTRTTHYGSLPQKSQHGRTQDE
  NPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQRP
  GFGYGGRASDYKSAHKGFKAYDAQGTLSKIFKLGGRDSR
  SGSPMARRLEHHHHHH
• BMRB code: 15131
• additional info:
  Solution NMR and CD spectroscopy of an intrinsically disordered, peripheral membrane protein: evaluation of aqueous and membrane-mimetic solvent conditions for studying the conformational adaptability of the 18.5 kDa isoform of myelin basic protein (MBP), European Biophysics Journal,Volume 37, Number 6 / July, 2008, 1015-1029

HSQC or other spectra:

new category–IDP–intrinsically disordered protein

I am trying to collect all NMR assigned IDPs and put the information in this blog. If you read this news and know something I don’t collect yet, please please write me a comment. It will be very very helpful to me and other guys they want to study IDPs.

Update (3/6/08):
I found that BMRB has a special collection called  “Unfolded Protein NMR Entries“. It ’s helpful but the entries are not many.

[IDP] osteopontin (OPN)

• Title:Backbone assignment of osteopontin, a cytokine and cell attachment protein implicated in tumorigenesis
• Journal: Biomolecular NMR Assignments
• DOI: 10.1007/s12104-007-9076-2
• Author:Andreas Schedlbauer, Przemyslaw Ozdowy, Georg Kontaxis, Markus Hartl, Klaus Bister and Robert Konrat
• number of AA:220
• Sequence:
  MHQDHVDSQSQEHLQQTQNDLASLQQTHYSSEENADVPEQ
  PDFPDVPSKSQETVDDDDDDDNDSNDTDESDEVFTDFPTE
  APVAPFNRGDNAGRGDSVAYGFRAKAHVVKASKIRKAARK
  LIEDDATTEDGDSQPAGLWWPKESREQNSRELPQHQSVEN
  DSRPKFDSREVDGGDSKASAGVDSRESQGSVPAVDASNQT
  LESAEDAEDRHSIENNEVTR
• BMRB code: 15519

HSQC or other spectra:

[IDP] Connexin40

• Title:¹H, ¹³C, and ¹⁵N backbone resonance assignments of the carboxyl terminal domain of Connexin40
• Journal: Biomolecular NMR Assignments
• DOI: 10.1007/s12104-007-9044-x
• Author:Denis Bouvier, Fabien Kieken and Paul L. Sorgen
• number of AA:109
• Sequence:

  GPLGSTSLVQ GLTPPPDFNQ CLKNSPDEKF FSDFSNNMGS RKNPDPLATE EVPNQEQIPE  60
  EGFIHTQYGQ KPEQPSGASA GHRFPQGYHS DKRRLSKASS KARSDDLSV              109
  

• BMRB code: 15266

HSQC or other spectra:

[IDP]ENSA- cAMP-regulated phosphoprotein endosulfine-alpha

• Title: ¹H, ¹³C, and ¹⁵N resonance assignment of the cAMP-regulated phosphoprotein endosulfine-alpha in free and micelle-bound states
• Journal: Biomolecular NMR Assignments
• DOI: 10.1007/s12104-007-9063-7
• Author:John M. Boettcher, Kevin L. Hartman, Daniel T. Ladror, Zhi Qi, Wendy S. Woods, Julia M. George and Chad M. Rienstra1
• number of AA:121
• Sequence:
  MSQKQEEENPAEETGEEKQDTQEKEGILPERAEEAKLKAK
  YPSLGQKPGGSDFLMKRLQKGQKYFDSGDYNMAKAKMKNK
  QLPSAGPDKNLVTGDHIPTPQDLPQRKSSLVTSKLAGGQVE
• BMRB code: 15136

HSQC or other spectra: